Journal: Cell reports

Article Title: Tuberculosis exacerbates HIV-1 infection through IL-10/STAT3-dependent tunneling nanotube formation in macrophages

doi: 10.1016/j.celrep.2019.02.091

Figure Lengend Snippet: (A) Vertical scatter plots showing the Median Fluorescence Intensity (MFI) of cell-surface receptors involved in HIV-1 entry (CD4, CCR5, CXCR4) on monocytes differentiated for 3 days under the presence of CmCTR and CmMTB. Each circle within vertical scatter plots represents a single donor. Mean value is represented as a dark grey line. (B) Histogram showing the percentage of HIV-1 fusion with CmCTR- (white) or CmMTB (black)-pre-treated cells, as determined using the Blam-Vpr assay in the presence of entry inhibitor Maraviroc (dashed bars). (C) Left: Representative images of Western Blot analysis illustrating the expression of IFITM1/2/3 and Actin as loading control. Right: Quantification of IFITM1/2/3 expressed as a ratio related to actin of monocytes differentiated for 3 days into macrophages under the presence of CmCTR (white) and CmMTB (black). n = 6 donors. (D) Representative images of Western Blot analysis illustrating the expression of SAMHD1 and its phosphorylated version (pSAMHD1), and Actin as loading control (left). Quantification of SAMHD1 (center) pSAMHD1 (right) expressed as a ratio related to actin of monocytes differentiated for 3 days into macrophages under the presence of CmCTR and CmMTB. n = 11 donors. (E) Representative images of Western Blot analysis illustrating the expression of C/EBP-β (LAP), C/EBP-β (LIP), and Actin as loading control (left). Quantification of C/EBP-β (LAP, center left) and C/EBP-β (LIP, center right) expressed as a ratio related to actin, and LAP expressed as a ratio related to LIP (right), of monocytes differentiated for 3 days into macrophages under the presence of CmCTR and CmMTB. n = 9 donors. LAP is an activator of HIV-1-LTR whereas LIP is a repressor of HIV-1-LTR. (F) Representative images of Western Blot analysis illustrating the expression of CUGBP1 and Actin as loading control (left). Quantification of CUGBP1 expressed as a ratio related to actin (right) of monocytes differentiated for 3 days into macrophages under the presence of CmCTR and CmMTB. n = 9 donors. (G) Quantification of LC3-II expression as a ratio to actin of monocytes differentiated for 3 days under the presence of CmCTR and CmMTB at the indicated time points after 2h treatment with Bafilomycin A1 (BafA1) or DMSO as control, as measured by western blot analysis. Uninfected cells at day 3 of the experiment (left, n = 6 donors), and HIV-infected cells at 1 (day 4, center, n = 4 donors) and 3 (day 6, right, n = 6 donors) days post-infection. Each circle within vertical scatter plots represents a single donor. Mean value is represented as a dark grey line. Data in histograms are represented as mean ± SD. * p≤0.05; ** p≤0.005; *** p≤0.0005; **** p≤0.0001.

Article Snippet: Anti-human phosphoThr592-SAMHD1 , ProSci , Cat# 8005.

Techniques: Fluorescence, Western Blot, Expressing, Infection

Journal: Cell reports

Article Title: Tuberculosis exacerbates HIV-1 infection through IL-10/STAT3-dependent tunneling nanotube formation in macrophages

doi: 10.1016/j.celrep.2019.02.091

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-human phosphoThr592-SAMHD1 , ProSci , Cat# 8005.

Techniques: Purification, Recombinant, Luciferase, Western Blot, Enzyme-linked Immunosorbent Assay, Fluorescence, Software, Imaging